bshB2 [2018-02-13T12:25:45.000Z]

N-acetylglucosamine-malate deacetylase, minor enzyme involved in bacillithiol synthesis, may act as amidase for the processing of BSH conjugates

Locus: BSU19460

Isoelectric point: 4.51

Molecular weight: 16.54 kDa

Protein length: 142 aa

Gene length: 426 bp

Function: biosynthesis of bacillithiol

Product: N-acetylglucosamin-malate deacetylase

Essential: no

Synonyms: yojG

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Miscellaneous metabolic pathways → Biosynthesis of bacillithiol
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

Gene

Coordinates: 2,121,641 → 2,122,306

The protein

Paralogous protein(s)

• BshB1

Expression and Regulation

Operons

• Genes: yoyC-yojF-bshB2
  Description: (major transcript) PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Spx: activation, PubMed, in Spx regulon

  Regulation

  • yoyC: induced by diamide stess (thiol depletion) (Spx) PubMed

• Genes: yojE-yoyC-yojF-bshB2
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • yoyC: induced by diamide stess (thiol depletion) (Spx) PubMed

Biological materials

Mutant

• MGNA-B428 (yojG::erm), available at the NBRP B. subtilis, Japan
• bshB2 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
• CS212 (bshB2::aphA3, available in Colin Harwood's and Jörg Stülke's labs) PubMed
• BKE19460 (ΔbshB2::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC
• BKK19460 (ΔbshB2::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTTCATTATTATTCACCCTT, downstream forward: _UP4_TAATCAAAAAGGTATAAGGC

References

Reviews

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Original Publications

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